Figure 7.

CHS and FLS are primary target genes of MYB12. A, Determination of PCR efficiencies in quantitative real time PCR. The example shows the determination for MYB12 as PCR target. The plot of the natural logarithm of template concentration against the threshold cycle number results in a linear standard curve. The efficiency of the PCR system used can be calculated from the slope of the graph. The equation and the corresponding correlation coefficient of the linear regression line is shown. The calculated efficiency value is also indicated. For details of the method see “Materials and Methods.” E, PCR efficiency; ln, natural logarithm; R², correlation coefficient of linear regression line. B, Results of quantitative real time RT-PCR analyses of RNA from developing Arabidopsis seedlings. Columns represent the relative expression levels of the distinct target genes tested. The mRNA amount in 2-d-old wild-type (WT) seedlings served as a calibrator for the calculation of relative expression levels in all cases (relative expression arbitrarily set to one). Relative expression was determined in triplicate measurements in two independent biological replicates. The age and the genotype of the seedlings analyzed are indicated.