FIG. 1.

Expression of IL-6 mRNA in various human and murine leukocyte populations. Primary cells were isolated as described in Materials and Methods. The primary cells and cell lines were seeded and left overnight to settle. The cells were infected with 3 × 10⁶ PFU of HSV-1 (KOS) per ml and stimulated with 10 IU of human or murine IFN-γ per ml. After 4 h of treatment, total RNA was isolated and subjected to RT-PCR using oligo(dT)₁₅ RT priming and PCR primers specific for murine β-actin, human IL-6, and murine IL-6. PBMC, peripheral blood mononuclear cells; PC, peritoneal cells; TG, thioglycolate.