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Construction of PEP-1–PIN1 and control PIN1 protein. Constructed map of PEP-1–PIN1 based on the pET-15b vector. PEP-1–PIN1 was designed to contain histidine, PEP-1-PTD and PIN1 (A). Purified PEP-1–PIN1 and control PIN1 were confirmed by Coomassie brilliant blue staining and Western blot analysis using anti-histidine antibody (B).
