Figure 3.

Effects of delivered PEP-1–PIN1 against H₂O₂-induced cell death. HT-22 cells were treated with PEP-1–PIN1 (5 μM) for 3 h. The localization of delivered PEP-1–PIN1 was confirmed by fluorescence microscopy (A). Scale bar = 20 μm. Effect of delivered PEP-1–PIN1 against H₂O₂-induced cell viability. The cells were pretreated with PEP-1–PIN1 (0.5–5 μM) for 3 h and exposed to H₂O₂ (1 mM) for 2 h. Cell viability was assessed by MTT assay (B). Data are represented as mean ± SEM (n = 3). * p < 0.05 and ** p < 0.01 compared with H₂O₂-treated cells.