Figure 6.

Effects of delivered PEP-1–PIN1 against H₂O₂-induced Bax, Bcl-2, and p53 protein expression in HT-22 cells. Three-hour pretreatment of HT-22 cells with PEP-1–PIN1 (5 μM) was followed by treatments with H₂O₂ (1 mM) for 120 min (Bcl-2), 240 min (Bax), and 360 min (p53). The expression levels of Bcl-2 and Bax (A) and p53 (B) were determined by Western blot analysis. The intensity of the bands was measured by a densitometer. Data are represented as mean ± SEM (n = 3). * p < 0.05 and ** p < 0.01 compared with H₂O₂ treated cells.