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Reaction mechanism and cyclization routes of CBT-Cys-like click reactions. (a) Reaction mechanism of CBT-Cys click reaction. Classical stimuli-induced cyclization routes of CBT-Cys (b), CHQ-Cys (c), and 2-pyrimidinecarbonitrile (PMN)-Cys (d) reactions. Usually, SR1, SR3, and SR5 represent the groups that are sensitive to reducing substances, and R2, R4, and R6 are peptide sequences that can be specifically cleaved by enzymes.
