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. 2023 Dec 31;2(2):98–116. doi: 10.1021/cbmi.3c00117

Figure 2.

Figure 2

(a) Schematic illustration of FL-“dual-quenched” Cy5.5-CBT-NPs preparation and cartoon representation of using Cy5.5-CBT-NPs to image the tumoricidal activity of cytotoxic T lymphocytes. (b) FL spectra of Cy5.5-CBT (10 μM, black), Cy5.5-CBT-NPs (5 μM, red), and Cy5.5-CBT-NPs (5 μM) treated with 20 pM GraB (blue) or 20 pM GraB + 100 μM GraB inhibitor (green) in tris(hydroxymethyl)aminomethane (Tris, 100 mM, pH 7.5) buffer for 4 h, λex = 675 nm, λem = 702 nm. (c) In vivo real-time FLI of B16-OVA tumor-bearing mice pretreated with saline (Control) or BEC (BEC treatment) for 3 days followed by intratumoral injection of 0.78 mg kg–1Cy5.5-CBT-NPs. (d) Quantification of FL signals in (c). ***P < 0.001. Reproduced with permission from ref (41). Copyright 2022 American Chemical Society.