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. 2023 Dec 31;2(2):98–116. doi: 10.1021/cbmi.3c00117

Figure 3.

Figure 3

(a) Schematic illustration of furin-controlled dual aggregation-induced emission for enhanced FL sensing of furin activity. (b) Fluorescence spectra of 100 μM 1-Ctrl (black) and 100 μM 1-Ctrl incubated with 1 nmol U–1 furin at 37 °C for 4 h in furin buffer (red), respectively (top row). Fluorescence spectra of 100 μM 1 (black) and 100 μM 1 incubated with 1 nmol U–1 furin at 37 °C for 4 h in furin buffer (red) (bottom row). Excitation wavelength: 320 nm. (c) Differential interference contrast images (top row), fluorescence images (middle row, DAPI channel), and merged images (bottom row) of MDA-MB-468 cells incubated with 5 μM 1 (left column) or 1-Ctrl (right column) coincubated with 50 μM Ac-Arg-Val-Arg-Arg-Cys(StBu)-Lys-CBT in a serum-free medium for 60 min at 37 °C and washed with PBS for three times prior to imaging, respectively. All images have the same scale bar: 10 μm. Reproduced with permission from ref (48). Copyright 2017 Royal Society of Chemistry.