FIG. 7.

RNA replication, protein synthesis, and virus production in Vero cells electroporated with AV-FL and mut6. (A) Dot blot analysis of plus-sense viral RNA accumulation in cells. Total RNAs were extracted from cells transfected with AV-FL and mut6 at the indicated time points after electroporation. The total RNA samples were dotted and probed with DIG-labeled minus-sense viral RNA. (B) Detection of capsid proteins by Western blotting. Cells were lysed at 3 and 9 h after electroporation with AV-FL and mut6. Each lysate was subjected to SDS–10% polyacrylamide gel electrophoresis, and capsid proteins were detected by Western blotting using antiserum raised against purified virus particles. As a control, purified virion was analyzed. Positions of VP0, VP1, and VP3 are indicated on the left. (C) Virus yields in cells electroporated with AV-FL and mut6. At the indicated time points after electroporation, cells were harvested and the virus titer was examined by plaque assay.