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. 2024 Oct 8;13(10):1211. doi: 10.3390/antiox13101211

Figure 2.

Figure 2

Zinc increased the activity of TXNRD but not of GPX. HepG2 cells were treated with or without 50 nM sodium selenite in combination with or without 100 µM zinc sulfate for up to 72 h (a–e,g,h) or with indicated Zn concentrations (f) in media containing 2.5% FCS. Enzyme activities of GPX (a) and TXNRD (e,f) and the protein expression levels of GPX1 (b), GPX2 (c), GPX4 (d), TXNRD1 (g), and TXNRD2 (h) were determined photometrically (a,e,f) or by Western blot (b–d,g,h). Protein expression was normalized to Ponceau staining and presented relative to +Se/+Zn treatment. Results are presented as mean + SD (n = 3–4). * p < 0.05, *** p < 0.001 vs. −Se, # p < 0.05, ## p < 0.01, ### p < 0.001 vs. −Zn, calculated by two-factorial ANOVA (a–e,g,h) or with one-factorial ANOVA (f) with Bonferroni’s post-test.