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. 2024 Oct 8;13(10):1211. doi: 10.3390/antiox13101211

Figure 5.

Figure 5

Trace element transporters were increased by zinc. HepG2 cells were treated with or without 50 nM sodium selenite for 72 h in combination with or without 100 μM zinc sulfate for indicated time (up to 72 h) in media containing 2.5% FCS. The mRNA expression levels of ZIP8 (a), XCT (b), and APOER2 (d) were analyzed by qPCR. Gene expression was normalized to the normalization factor of the reference genes HPRT, RPL13a, and GAPDH and was presented relative to samples with selenium and 6 h zinc treatment. XCT (c) and APOER2 (e) were analyzed by Western blot. Protein expression was normalized to Ponceau staining and presented relative to samples with selenium and 48 h (c) or 72 h zinc treatment (e). Results are presented as mean + SD (n = 3–4). * p < 0.05 vs. −Se; # p < 0.05, ## p < 0.01, ### p < 0.001 vs. −Zn, $$$ p < 0.001 vs. 6 h Zn, calculated by two-factorial ANOVA (a,b,d) or with one-factorial ANOVA (c,e) with Bonferroni’s post-test.