Figure 8.

Synthetic Kalata B1 increased TMZ-induced cell death in U-87 MG glioblastoma cultures. To determine if synthetic Kalata B1 (K) enhances TMZ (T)-induced cell death in U-87 MG cells, MTT was measured after incubating the cell cultures with concentrations of K (0.5, 1.0, 2.0, and 4.0 μM) and T (100, 200, 400, and 800 μM) both alone and when co-exposed to synthetic Kalata B1 and TMZ as illustrated in panels (A–M). The first dotted bars in each panel indicate the cells exposed to concentrations of synthetic Kalata B1 alone, while the second diagonal stripe bars indicate the cells exposed to TMZ alone. The third checkered bars indicate co-exposure treatments with synthetic Kalata B1 and TMZ. The cells were plated into 96-well plates (6000 cells/well) and treated with synthetic Kalata B, as indicated, for 72 h. Cell viability was measured using MTT and expressed as a percentage of control cultures (culture medium with DMSO only). Incubations were conducted in triplicate wells (technical replicates) and as three independent incubations (biological replicates, n = 3). Data are expressed as mean ± standard error of the mean (SEM). Results of one-way analysis of variance (ANOVA) and Tukey’s multiple comparisons are graphed in GraphPad Prism version 9 for iOS with the following p-values: * < 0.05, ** < 0.01, *** < 0.001, and **** < 0.0001. ns = not significant.