TABLE 1.
Effect of poly(I-C) on viral RNA levels for tamarin hepatocytes infected with GBV-B
| Poly(I-C)a | Sample | Viral RNA level (log10 ge) at passage no.b:
|
||
|---|---|---|---|---|
| 0 | 1 | 2 | ||
| + | Cells | 3.43 | 1.11 | 0 |
| Medium | 3.18 | 0 | 0 | |
| − | Cells | 5.65 | 5.11 | 5.00 |
| Medium | 5.61 | 4.80 | 4.62 | |
a
Tamarin hepatocytes were cultured in the presence (+) or absence (−) of 50 μg of poly(I-C) per ml starting 1 day before infection with GBV-B and for 7 days after infection.
b
Cultures were harvested for determination of viral RNA levels (log10 ge, log10 value of genome equivalent per microgram of cell RNA or genome equivalents per milliliter of medium). The culture medium from day 7 was used to inoculate fresh cultures (passage 1). The process was repeated a second time (passage 2).