Figure 1.

Flow cytometry analysis of CD4⁺ T helper cells, with (A) a representative gating strategy for a PBMC sample. The percentages of (B) CD4⁺ T cells among single lymphocytes were analyzed by flow cytometry, as well as the proportions of (C) CD25⁺CD127^low, (D) CD25⁺CD127^lowFoxP3⁺ and (F) PD-1⁺ cells among all CD4⁺ T cells. (E) The FoxP3 MFI for the CD25⁺CD127^low cells was also measured. PBMCs = peripheral blood mononuclear cells. Cryo = cryopreserved. MFI = median fluorescence intensity. ns=non-significant. Paired non-parametric Friedman test, followed by Dunn’s multiple comparisons test; n = 10 for (B,F) and n = 9 for (C–E). * indicates p < 0.05 and ** p < 0.01 (adjusted p-values).