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. 2024 Oct 11;12(10):2319. doi: 10.3390/biomedicines12102319

Figure 3.

Figure 3

Flow cytometry analysis of CD8+ T cytotoxic cells with (A) a representative gating strategy for a PBMC sample. The percentages of (B) CD8+ T cells among single lymphocytes were analyzed by flow cytometry, as well as the proportions of (C) naive/stem cell memory cells (CD45RA+CCR7+), (D) central memory (CD45RA−CCR7+), (E) effector memory (CD45RA−CCR7−), (F) TEMRA (CD45RA+CCR7−) and (G) PD-1+ cells among all CD8+ T cells. PBMCs = peripheral blood mononuclear cells. Cryo = cryopreserved. ns=non-significant. Paired non-parametric Friedman test, followed by Dunn’s multiple comparisons test, n = 10. * Indicates p < 0.05 and ** p < 0.01 (adjusted p-values).