Table 1.
Monoclonal antibodies used for the staining and flow cytometric analysis of different peripheral immune cells and their subsets.
| Cell Populations | Antibody * | Clone | Titration | Fluorochrome |
|---|---|---|---|---|
| CD4+ T helper and T regulatory cells | CD4 | SK3 | 1:2 | PerCP-Cy5.5 |
| CD25 | M-A251 | 1:2 | PE-Cy7 | |
| CD26 | M-A261 | 1:2 | BV510 | |
| CD122 | Mik-β3 | 1:4 | APC | |
| CD127 | HIL-7R-M21 | 1:2 | BV421 | |
| FoxP3 | 236A/E7 | 1:1 | PE | |
| PD-1 (CD279) | EH12.1 | 1:1 | BB515 | |
| CD4+ T helper cell subpopulations | CD3 | UCHT1 | 1:2 | FITC |
| CD4 | RPA-T4 | 1:2 | APC-Cy7 | |
| CD25 | M-A251 | 1:2 | PE | |
| CD45RO | UCHL1 | 1:2 | PerCP-Cy5.5 | |
| CD127 | HIL-7R-M21 | 1:4 | Alexa Fluor 647 | |
| CCR4 (CD194) | 1G1 | 1:2 | BV510 | |
| CCR5 (CD195) | 2D7 | 1:4 | APC | |
| CCR6 (CD196) | 11A9 | 1:4 | PE-Cy7 | |
| CCR10 | 1B5 | 1:1 | PE | |
| CXCR3 (CD183) | 1C6 | 1:2 | BV421 | |
| CD8+ T cytotoxic cells | CD8 | SK1 | 1:2 | APC-H7 |
| CD25 | M-A251 | 1:2 | PE-Cy7 | |
| CD26 | M-A261 | 1:2 | BV510 | |
| CD39 | TU66 | 1:2 | FITC | |
| CD45RA | HI100 | 1:4 | PerCP-Cy5.5 | |
| CD122 | Mik-β3 | 1:2 | APC | |
| CD127 | HIL-7R-M21 | 1:2 | BV510 | |
| CCR7 (CD197) | 150503 | 1:2 | Alexa Fluor 647 | |
| FoxP3 | 236A/E7 | 1:1 | PE | |
| PD-1 (CD279) | EH12.1 | 1:1 | BB515 | |
| Perforin | δG9 | 1:1 | BV421 | |
| B cells, NK cells and monocytes | CD1a | HI149 | 1:2 | APC |
| CD3 | SK7 | 1:2 | FITC | |
| CD11c | 3.9 | 1:1 | BV421 | |
| CD14 | MφP9 | 1:2 | APC-H7 | |
| CD19 | SJ25C1 | 1:2 | PerCP-Cy5.5 | |
| CD16 | 3G8 | 1:2 | PE-Cy7 | |
| CD56 | NCAM16.2 | 1:1 | PE | |
| CD123 | 9F5 | 1:1 | BV510 |
* All antibodies were manufactured by BD Biosciences. APC = allophycocyanin; BB = Brilliant Blue™; BV = Brilliant Violet™; Cy = cyanine; FITC = fluorescein-isothiocyanate; PE = phycoerythrin; PerCP = peridinin-chlorophyll-protein.