Figure 5.
Effects of ATRA on 3D spheroid configuration of 3D ARPE19 spheroids not treated or treated with TGF-β2 under normoxia and hypoxia conditions. In the absence or presence of ATRA (100 nM), 3D ARPE19 spheroids not treated (Ctrl) or treated with TGF-β2 (5 ng/mL) were prepared under normoxia (20% O2) and hypoxia (1% O2) conditions. Representative phase contrast downward images ((A): day 1 under normoxia, (B): day 2 under normoxia, (C): day 3 under normoxia, (D): day 4, under normoxia) (E): day 5 under normoxia, (F): day 6 under normoxia, (G): day 6 under hypoxia) and lateral images ((H): day 6 under normoxia) are shown. Scale bar: 100 μm and 50 μm.