TABLE 2.
Viral transcripts detected in HSV viral particles by RT-PCR and HSV arraya
| Gene(s) | RT-PCR
|
Array, HSV-1
|
||||
|---|---|---|---|---|---|---|
| HSV-1
|
R7023
|
|||||
| Vero/I | HEp-2/I | HEp-2/E | HEp-2/E | HEp-2/E | Position | |
| UL1 | − | − | − | − | − | A1 |
| UL2 | − | − | − | − | − | A2 |
| UL3 | − | − | − | − | − | A3 |
| UL4 | − | − | − | − | + | A4 |
| UL5 | − | − | − | − | − | A5 |
| UL6 | − | − | − | − | − | A6 |
| UL7 | + | − | − | + | − | A7 |
| UL8 | − | − | − | − | − | A8 |
| UL8.5/9 | − | − | − | − | − | A9 |
| UL9 | − | − | − | − | − | A10 |
| UL9.5/10 | + | + | + | + | + | A11 |
| UL11 | − | − | − | − | + | A12 |
| UL12 | + | − | − | + | + | A13 |
| UL13 | + | − | + | + | − | A14 |
| UL14 | − | − | − | − | − | A15 |
| UL15 | + | − | − | − | − | B1 |
| UL15-2/15.5 | ND | ND | ND | ND | − | B2 |
| UL16 | − | − | − | − | − | B3 |
| UL17 | − | − | − | − | − | B4 |
| UL18 | − | − | − | + | + | B5 |
| UL19-1 | + | − | + | + | + | B6 |
| UL19-2 | ND | ND | ND | ND | − | B7 |
| UL20 | + | − | − | − | − | B8 |
| UL20.5 | − | − | − | + | − | B9 |
| UL21 | + | − | + | + | − | B10 |
| UL22 | − | − | − | − | − | B11 |
| UL23 | − | − | − | − | − | B12 |
| UL24 | − | − | − | − | − | B13 |
| UL25 | − | − | − | − | − | B14 |
| UL26 | ND | ND | ND | ND | − | B15 |
| UL26/26.5 | − | − | − | − | − | C1 |
| UL27 | + | + | − | − | − | C2 |
| UL27.5/28 | − | − | − | − | − | C3 |
| UL28 | ND | ND | ND | ND | − | C4 |
| UL29-1 | + | − | + | + | − | C5 |
| UL29-2 | ND | ND | ND | ND | − | C6 |
| UL30-1 | − | − | − | − | + | C7 |
| UL30-2 | ND | ND | ND | − | C8 | |
| UL31 | + | − | − | − | − | C9 |
| UL32 | − | − | − | − | − | C10 |
| UL33 | − | − | − | − | − | C11 |
| UL34 | + | + | + | + | − | C12 |
| UL34/35 | + | + | + | + | + | C13 |
| UL36-1 | + | + | + | + | + | C14 |
| UL36-2 | ND | ND | ND | ND | + | C15 |
| UL36-3 | ND | ND | ND | ND | − | D1 |
| UL37 | + | − | + | − | − | D2 |
| UL38 | − | − | − | − | − | D3 |
| UL39 | − | − | − | − | − | D4 |
| UL40 | + | + | − | + | − | D5 |
| UL41 | − | − | − | − | − | D6 |
| UL42 | + | + | + | + | − | D7 |
| UL43 | ND | ND | ND | ND | − | D8 |
| UL43/43.5 | − | − | − | − | − | D9 |
| UL44 | − | − | − | − | + | D10 |
| UL45 | + | − | − | − | − | D11 |
| UL46 | − | − | − | − | + | D12 |
| UL47 | − | − | − | − | − | D13 |
| UL48 | + | + | + | + | + | D14 |
| UL49 | − | − | − | − | + | D15 |
| UL49.5 | − | − | + | − | − | E1 |
| UL50 | − | − | − | − | − | E2 |
| UL51 | + | + | + | + | + | E3 |
| UL52 | − | − | − | − | − | E4 |
| UL53 | − | − | − | − | − | E5 |
| UL54 | − | − | − | − | − | E6 |
| UL55 | + | − | + | − | − | E7 |
| UL56 | − | − | + | − | − | E8 |
| α0-1 | ND | ND | ND | ND | + | E9 |
| α0-2 | − | − | − | − | + | E10 |
| α4-1 | + | − | + | + | + | E11 |
| α4-2 | ND | ND | ND | ND | − | E12 |
| LAT/ORFO γ1 34.5 | − | − | − | − | − | E13 |
| Oris-1 | − | − | − | − | − | E14 |
| Oris-2 | ND | ND | ND | ND | − | E15 |
| US1 | ND | ND | ND | ND | − | F1 |
| US1/1.5 | + | + | + | + | − | F2 |
| US2 | − | − | − | − | − | F3 |
| US3 | − | − | − | − | + | F4 |
| US4 | + | + | − | + | + | F5 |
| US5 | − | − | − | − | − | F6 |
| US6 | − | − | − | − | − | F7 |
| US7 | + | − | + | − | − | F8 |
| US8 | − | − | − | − | − | F9 |
| US8.5 | + | + | + | − | + | F10 |
| US9 | − | − | − | − | + | F11 |
| US10/11 | + | + | + | − | + | F12 |
| US11 | + | + | + | − | − | F13 |
| US12 | − | − | − | − | + | F14 |
| US12-2 | − | − | − | − | ND | |
a
Vero/I and HEp-2/I, virions purified from homogenized infected cells; HEp-2/E, virions purified from supernatant. R7023 is an HSV-1 mutant lacking the genes US8 through US12. Two or three pairs of primers were designed for the genes that contain exons (i.e., α0, UL15) or for the ORFs longer than 3.5 kb (i.e., UL19-1, UL29, UL30, UL36, α4). The position of the ORFs in the HSV array is also reported (see Fig. 2). ND, not done.