Table 1.
Comparison of biochemical parameters for ALO family members characterized from different species.
| Species | Molecular Mass (kDa) | Optimum pH | Optimum Temperature (°C) | Km (mM) | Vmax (µmol/min/mg Protein) | Reference |
|---|---|---|---|---|---|---|
| Rat liver GULO | 51 | 7.8 | 37 | 0.066 | 0.63 | [3] |
| Goat liver GULO | 51 | Nd | Nd | 0.15 | 2.7 | [3] |
| Chicken kidney GULO | 50 | 7.3 | Nd | 0.007 | 139 | [87] |
| Recombinant fGULO expressed in E. coli | 50 | 7 | 40 | 0.053 | 780 | [110] |
| Recombinant cGULO expressed in E. coli | 20 | 6.5 | 30 | 0.042 | 374 | [110] |
| GfGULLO | 69 | 7 | 45 | 24 | 2.1 | [99] |
| MtGLDH | 70 | 8 | 40 | 5.5 | 0.041 | [105] |
| AtGalDH | 60 | 8.8 | 25 | 13.1 | Nd | [59] |
| MsGULLO | 70 | Nd | Nd | Nd | Nd | [13] |
| Recombinant (AtGULLO5) expressed in N. benthamiana | 65 | 9 | 40 | 33.8 | 4.5 × 10−3 | [43] |
Nd, not determined; fGULO, rat full-length L-gulono-γ-lactone oxidase; cGULO, rat C-terminal L-gulono-γ-lactone oxidase; GfGULLO, Grifola frondosa L-gulono-γ-lactone oxidase; MtGLDH, Mycobacterium tuberculosis L-gulono-γ-lactone dehydrogenase; AtGalDH, Arabidopsis thaliana L-galactono-γ-lactone dehydrogenase; MsGULLO, Mucuna sempervirens L-gulono-γ-lactone oxidase; AtGULLO5, Arabidopsis thaliana homologue of GULLO.