Table 2.
Primer sequences and PCR conditions for sequencing analysis
| Segment | Size (bp) | Nucleotide position | Gene included | Primers (5′–3′) | PCR conditions |
|---|---|---|---|---|---|
| ND1 | 1,463 | 3,187–4,650 | 16s rRNA, ND1, ND2 |
F: CTCAACTTAGTATTATACCC R: GGAAATACTTGATGGCAGCT |
95: 50″, 58: 55″, 72: 50″ (30 cycle); 72: 10′ |
| ND2 | 1,249 | 4,491–5,740 | ND2 |
F: GTCATCTACTCTACCTACTT R: GGCGGGAGAAGTAGATTGAA |
95: 55″, 56.5: 1′, 72: 55″ (35 cycle); 72: 10′ |
| ND3 | 1,499 | 10,361–11,860 | ND3, ND4L, ND4 |
F: TCTGGCCTATGAGTGACTAC R: GAGGTTAGCGAGGCTTGCTA |
95: 55″, 58.5: 1′, 72: 55″ (30 cycle); 72: 10′ |
| ND4 | 519 | 11,901–12,420 | ND4, ND5 |
F: TGCTAGTAACCACGTTCTCC R: TTTGTTAGGGTTAACGAGGG |
95: 50″, 57: 1′, 72: 55″ (30 cycle); 72: 10′ |
| ND5 | 1,334 | 13,506–14,840 | ND5, ND6 |
F: TCCAAAGACCACATCATCGAAAC R: TTCATCATGCGGAGATGTTG |
95: 55″, 59: 1′, 72: 55″ (30 cycle); 72: 10′ |