Table 2.

Guiding questions for this SR on the use of CRISPR/Cas technology to edit tolerance genes/resistance to biotic stresses from studies published in the last 12 years.

Research Questions
1. In which country was the study performed?
2. What culture is the article about?
3. Which biotic agent is addressed in the study?
4. Which genes are reportedly associated with disease and pest resistance in plants?
5. Which nuclease is used in conjunction with the CRISPR tool?
6. What methodology is used to use CRISPR?
7. What method is used to prove the effectiveness of the tool?
8. What techniques/tools are associated with CRISPR/Cas9?
9. What transformation method was used?
10. Which explant was used to transform the plants?
11. What are the main vectors used to express Cas9 and/or gRNA in plants?
12. Were any unusual phenotypic characteristics observed in the plants after genetic transformation? Which ones?
13. What is the characteristic obtained after mutating the plant?