Figure 4.

Effects of β2-AR knockdown or overexpression on the expression of TNFα in LPS-treated RAW264.7 cells treated with Ad. Cells were transfected with the specific siRNA targeting β2-AR (β2-AR-siRNA) and control siRNA (Con-siRNA), and plasmid overexpressing β2-AR (pcDNA-β2-AR) and its control (pcDNA), respectively. (a,c) β2-AR expression was then examined via immunoblotting. (b,d) Transfected cells were treated with LPS with or without Ad for 2 h, and TNFα protein levels were determined (upper panel). One representative result from three independent experiments is shown. GAPDH was used as a loading control. The band density was quantitatively analyzed using ImageJ software. The results (lower panel in (b,d)) are expressed as mean ± SE (n = 3). *** p < 0.001; # p < 0.05 and ### p < 0.001; ††† p < 0.001.