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. 2021 Sep 17;42(42):4373–4385. doi: 10.1093/eurheartj/ehab605

Figure 3.

Figure 3

Inhibition of oxoglutarate dehydrogenase (OGDH) reduces the expression of inflammatory factors in macrophages. (A) Schematic diagram of the tricarboxylic acid cycle. B, Representative immunofluorescence staining for F4/80 and OGDH in the suprarenal abdominal aortas from NaCl- and angiotensin II-infused mice. Scale bar = 50 μm. Arrows indicate macrophages expressing OGDH. (C and D) Differentially expressed genes were validated by qRT-PCR in bone marrow-derived macrophages stimulated with lipopolysaccharide after transfection with negative control (NC) or siOGDH. Data are pooled from three independent experiments. n = 9 per group. (E) Representative western blot of pro-IL-1β in lipopolysaccharide-stimulated bone marrow-derived macrophages after transfection with NC or siOGDH. (F) Relative succinate abundance in lipopolysaccharide-stimulated bone marrow-derived macrophages after transfection with NC or siOGDH. Data are pooled from three independent experiments. n = 9 per group. (G and H) Differentially expressed genes were validated by qRT-PCR in bone marrow-derived macrophages stimulated with lipopolysaccharide with or without the addition of succinyl phosphonate. Data are pooled from three independent experiments. n = 9 per group (G and H). (I) Representative western blot of pro-IL-1β in bone marrow-derived macrophages stimulated with lipopolysaccharide with or without the addition of succinyl phosphonate.