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. 2021 Sep 17;42(42):4373–4385. doi: 10.1093/eurheartj/ehab605

Figure 4.

Figure 4

Cyclic AMP-responsive element-binding protein 1 (CREB) regulates oxoglutarate dehydrogenase OGDH expression via direct promoter binding. (A) CREB-binding motifs. (B) Luciferase reporter analysis in 293T cells. Results are presented as the mean ± standard deviation. Data are pooled from three independent experiments. n = 11 per group. (C) Chromatin immunoprecipitation-seq assay of the occupancy of OGDH gene promoters by CREB (data from GSM2663863 dataset). (D) Chromatin immunoprecipitation assay: binding of CREB to the OGDH gene promoter in bone marrow-derived macrophages. Three independent experiments were performed. (E) Representative western blot of OGDH in bone marrow-derived macrophages stimulated with lipopolysaccharide for 24 h with or without addition of siCREB. Three independent experiments were performed. (F) Representative immunofluorescence staining for F4/80 and phosphorylated CREB in the suprarenal abdominal aortas from control and aortic aneurysm and dissection mice. Scale bar = 50 μm.