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. 2021 Sep 17;42(42):4373–4385. doi: 10.1093/eurheartj/ehab605

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Published on behalf of the European Society of Cardiology. All rights reserved. © The Author(s) 2021. For permissions, please email: journals.permissions@oup.com.

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p38α affects macrophage inflammatory phenotype by regulating the expression of OGDH and succinate via CREB phosphorylation. (A) Representative western blot of phosphorylated p38α (p-p38α), total p38α, p-CREB, and total CREB in p38α^fl/fl and p38α^MKO bone marrow-derived macrophages stimulated with lipopolysaccharide for 0, 7.5, 15, 30, 60, or 120 min. (B) The co-immunoprecipitation assay of CREB and p38α in bone marrow-derived macrophages. (C) Oxoglutarate dehydrogenase (Ogdh) mRNA level was measured by qRT-PCR in p38α^fl/fl and p38α^MKO bone marrow-derived macrophages stimulated with lipopolysaccharide. Data are pooled from three independent experiments. n = 9 per group. (D) Representative western blot of OGDH in p38α^fl/fl and p38α^MKO bone marrow-derived macrophages stimulated with lipopolysaccharide. (E) Relative succinate abundance in p38α^fl/fl and p38α^MKO bone marrow-derived macrophages stimulated with lipopolysaccharide. Data are pooled from three independent experiments. n = 9 per group. (F) Representative western blot of pro-IL-1β in p38α^fl/fl and p38α^MKO bone marrow-derived macrophages stimulated with lipopolysaccharide. (G) Expression levels of Nos2, Il1b, and Ccl2 determined by qRT-PCR in p38α^fl/fl and p38α^MKO bone marrow-derived macrophages stimulated with lipopolysaccharide. (H) Percentage of CD86⁺ cells determined by flow cytometry in p38α^fl/fl and p38α^MKO bone marrow-derived macrophages stimulated with lipopolysaccharide. Data are pooled from three independent experiments. n = 9 per group. (I) Dihydroethidium staining of reactive oxygen species in p38α^fl/fl and p38α^MKO bone marrow-derived macrophages stimulated with lipopolysaccharide. Scale bar = 50 μm.