Protein expression of matrix metalloproteinases (MMPs) in 3D HCFs and HKCs after being stimulated with 1 ng/mL, 4 ng/mL, and 8 ng/mL GnRH for 4 weeks. Protein expression of (A) MMP-1, (B) MMP-2, and (C) MMP-9 in HCFs and HKCs stimulated with GnRH. Statistical significance based on a one-way ANOVA with Šídák’s multiple comparisons test. Post hoc significance indicated as * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001. Statistical significance between HCFs and HKCs within the dose determined by the unpaired t-test with Welch’s correction. Significance indicated as # p < 0.05, ## p < 0.01, and ### p < 0.001.