Table 1.
Western blot antibodies used in 2D and 3D cell culture. Adapted with permission from Escandon et al., 2023 [40]. American Society for Investigative Pathology.
| Name | Source | Reactive | Dilution | Study | Antibody |
|---|---|---|---|---|---|
| LHR | ab125214 (Abcam, Cambridge, MA, USA) | Rabbit polyclonal | 1:500 | 3D cell culture | Primary |
| FSHR | ab75200 (Abcam, Cambridge, MA, USA) | Rabbit polyclonal | 1:500 | 3D cell culture | Primary |
| GnRHR | ab183079 (Abcam, Cambridge, MA, USA) | Rabbit polyclonal | 1:500 | 2D + 3D cell culture | Primary |
| MMP-1 | ab38929 (Abcam, Cambridge, MA, USA) | Rabbit polyclonal | 1:500 | 3D cell culture | Primary |
| MMP-2 | ab181286 (Abcam, Cambridge, MA, USA) | Rabbit monoclonal | 1:500 | 3D cell culture | Primary |
| MMP-9 | ab38898 (Abcam, Cambridge, MA, USA) | Rabbit polyclonal | 1:500 | 3D cell culture | Primary |
| α-SMA | ab5694 (Abcam, Cambridge, MA, USA) | Rabbit polyclonal | 1:500 | 3D cell culture | Primary |
| EDA-Fn | SAB4200784 (Millipore Sigma, Burlington, MA, USA) | Mouse monoclonal | 1:700 | 3D cell culture | Primary |
| β-actin | ab184092 (Abcam, Cambridge, MA, USA) | Mouse monoclonal | 1:2000 | 2D + 3D cell culture | Primary |
| AlexaFluor 568 | ab175470 (Abcam, Cambridge, MA, USA) | Anti-rabbit IgG | 1:2000 | 2D + 3D cell culture | Secondary |
| AlexaFluor 647 | ab150107 (Abcam, Cambridge, MA, USA) | Anti-mouse IgG | 1:2000 | 3D cell culture | Secondary |