Fig. 5.

Effects of fasudil mesylate (FM) on the viability of rat brain cortical and hippocampal slices after OGD insult in vitro. OD₄₉₀ value of TTC staining (A) was decreased but LDH efflux (B) increased in OGD group, whereas fasudil mesylate (10, 100 μmol/l) significantly attenuated these changes (P < 0.05). The data are expressed as mean ± SEM (n = 8). Compared with the control group, fluorescent intensity of PI staining (C) was significantly increased in OGD group, whereas fasudil mesylate at both 10 and 100 μmol/l decreased PI fluorescent intensity of cortical and hippocampal slices after OGD insult (P < 0.05). The data are expressed as mean ± SEM. (n = 5). For (A–C), *** P < 0.001 vs. control group; ^#  P < 0.05, ^##  P < 0.01, and ^###  P < 0.001 vs. OGD group. In the bottom are representative images of PI staining (D) for cortical and hippocampal slices (×10). These graphs are (a and e) control slices without OGD; (b and f) OGD slices; (c and g) OGD slices + FM 10 μM; (d and h) OGD slices + FM 100 μM