Figure 2.

m⁶A modifications are involved in the regulation of muscle development. (A) qPCR experiment detected the expression level of m⁶A-related genes in muscle of NX and LW pigs. (B) Representative FTO (green) of longissimus dorsi sections from NX and LW pigs; DAPI was used for nuclear staining (blue) (scale bar: 100 μm). (C) Western blotting result showed elevated levels of FTO expression and decreased levels of METTL4 expression in LW pig muscle. (D) Quantification of m⁶A levels was performed with ImageJ. (E) Western blotting showed that the protein expression of FTO was significantly escalated in porcine satellite cells (PSCs) on days 0, 2, and 4 post-differentiation. (F) Immunofluorescence staining of Myhc showed that the proportion of Myhc+ cells was significantly increased by FTO overexpression and significantly decreased by FTO knockdown (n = 3). * p < 0.05, ** p < 0.01.