Figure 7.

m⁶A modification regulated differential expression of WFS1 in NX and LW pig muscle. (A) Genome browser tracks showing RNA-seq (grey) and MeRIP-seq (blue and green) data at WFS1 gene loci. (B) MeRIP-qPCR validated the m⁶A modification of WFS1. IgG was used as negative control. (C) Detection of WFS1 expression in LW and NX pig muscle. (D) RT-PCR analysis of WFS1 mRNA expression in PSCs treated with FB23-2 or DMSO. (E) Detection of mRNA degradation rate of WFS1 in PSCs treated with FB23-2 or DMSO. (F) Detection of WFS1 expression in DMSO- and FB23-2-treated groups after interference with YTHDF2. (G) Sequencing results of these six pigs at the m⁶A binding peak sequence and the SNP site display. (H) Dual fluorescence detection of SNP sites on m⁶A binding peaks. The binding fragments containing C or T were cloned into the pmirGLO plasmid, named pmirGLO-MutC and pmirGLO-MutT, respectively (up). Then, they were transfected into PSCs along with the negative control empty vector (pmirGLO-WT), followed by treatment with FB23-2 (red +/−). * p < 0.05, ** p < 0.01.