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. 2024 Oct 25;10(43):eadq0101. doi: 10.1126/sciadv.adq0101

Fig. 3. Endocytosis pathway of TgMIF and its interaction with CD74.

Fig. 3.

(A) Effect of endocytosis inhibitors (10 mM methyl-β-cyclodextrin, cholesterol chelator; 1 μM amiloride, inhibits Na+/H+ exchange; 10 μM cytochalasin D, inhibitor of actin polymerization) on TgMIF expression in BMDMs. MFI, mean fluorescence intensity; DMSO, dimethyl sulfoxide. (B) Inhibition of TgMIF expression by shCD74 treatment in BMDMs. (C) Binding specificity of the TgMIF peptide (1 μM) with CD74. (D) Inflammatory responses of TgMIF peptide (1 μM) in BMDMs. The data are representative of four independent experiments with similar results [(B) and (C)]. Statistical significance was determined by the Student’s t test with Bonferroni adjustment (*P < 0.05; ***P < 0.001). Full-length images of the blots presented in fig. S8.