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. 2024 Oct 25;10(43):eadq0101. doi: 10.1126/sciadv.adq0101

Fig. 4. TgMIF modulates mitochondrial dynamics and metabolism through AZIN1.

Fig. 4.

(A to C) AZIN1-dependent regulation of mitochondrial fission and fusion gene expression by TgMIF (1, 5, and 10 μM). (D and E) AZIN1-dependent modulation of ATP synthase–related gene expression by TgMIF. (F) Cellular ATP production in MTB-infected BMDMs is dependent on TgMIF and AZIN1. (G) Restoration of mitochondrial respiration and ECARs by MTB infection with TgMIF and AZIN1. Oligo, oligomycin; FCCP, carbonyl cyanide p-trifluoromethoxyphenylhydrazone; 2-DG, 2-deoxyglucose. (H) Measurement of mtDNA content and mitochondria complex V activity in MTB-infected macrophages. (I) Number of CFU of MTB. Statistical significance was determined by the Student’s t test with Bonferroni adjustment (*P < 0.05; **P < 0.01; ***P < 0.001). ns, not significant. Full-length images of the blots presented in fig. S8.