Figure 1.

(A–D) Effect of systemic β3-AR agonist (CL 316243) administration (0.1 and 1 mg/kg) on IBAT temperature (T_IBAT), energy intake and body weight post-sham or IBAT denervation in female HFD-fed rats. Rats were maintained on HFD (60% kcal from fat; N = 4–8/group) for approximately 4.5 months prior to undergoing a sham or bilateral surgical IBAT denervation and implantation of temperature transponders underneath IBAT. Animals were subsequently adapted to a 4-h fast prior to receiving IP injections of CL 316243 (0.1 or 1 mg/kg, IP) or vehicle (sterile water) where each animal received each treatment at approximately 7-day intervals. (A,B) Effect of CL 316243 on T_IBAT in (A) sham operated or (B) IBAT denervated DIO rats; (C) Effect of CL 316243 on change in energy intake in sham or IBAT denervated DIO rats; (D) Effect of CL 316243 on change in body weight in sham or IBAT denervated DIO rats. Data are expressed as mean ± SEM. * p < 0.05 CL 316243 vs. vehicle.