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. 2024 Oct 12;13(20):1686. doi: 10.3390/cells13201686

Figure 1.

Figure 1

Human hematopoietic engraftment and T-cell development in humanized NRG mice used as a reference strain. (A) Scheme of experiments: CD34+ stem cell transplantation (HCT) i.v. after irradiation, blood collection (BL), and termination (X). The humanized mice were humanized with three different CB donors: #396 (depicted as a triangle), #395 (depicted as a square), and #376 (depicted as a circle). Both female and male mice were used in this experiment (details in Table S1). Created in BioRender. Lab, S. (2024) BioRender.com/x52w302. (B) Blood analyses at weeks 8, 12, and 20 after HCT and longitudinal quantification of cells expressing huCD45, huCD34, huCD19, huCD3, huCD4, and huCD8 (in percentages). (C) Bone-marrow analyses showing the quantification of cells expressing huCD45, huCD34, huCD3, huCD4, huCD8, and double positive (DP) (in absolute cell counts, log scale). (D) Thymus analyses and the quantification of cells expressing huCD45, huCD34, huCD3, huCD4, huCD8, and DP (in absolute cell counts, log scale). (E) Spleen analyses and quantification of cells expressing huCD45, huCD34, huCD3, huCD4, huCD8, and DP (in absolute cell counts, log scale).