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PCR analysis of rOkaΔORF64, rOkaΔORF69, and rOkaΔORF64/69. PCR analysis was done using cosmid DNA (lanes 1 to 4) or DNA isolated from infected cells (lanes 6 to 11). Results obtained with the ORF64 primers (A) and with the ORF69 primers (B) are shown. Lanes 1, pvSpe21ΔAvr; lanes 2, pvSpe21ΔORF64; lanes 3, pvSpe21ΔORF69; lanes 4, pvSpe21ΔORF64/69; lanes 5 and 12, 1-kb DNA ladder; lanes 6, rOka; lanes 7 and 11, rOkaΔORF69; lanes 8 and 10, rOkaΔORF64; lanes 9, rOkaΔORF64/69.
