TABLE 1.

Infectivities of cosmids with deletions of ORF63, ORF70, or ORF63/70

Cosmid cotransfected with pvFsp4, pvSpe5, and pvPme19	CPEa	Days post-transfection	Designation
pvSpe21ΔAvr	+++	8–10	rOka
pvSpe21ΔORF63	+++	8–10	rOkaΔORF63
pvSpe21ΔORF70	+++	8–10	rOkaΔORF70
pvSpe21ΔORF63/70	−	28–30b	NAc
pvSpe21ΔORF63/70+63@Avr	+++	8–10 days	rOka/ORF63rev

^aSymbols indicate proportions of cells exhibiting altered morphology: +, 25 to 50%; ++, 50 to 75%; +++, 75 to 90%; −, no CPE detected. 

^bCells were split every 4 to 5 days to ensure that a slow-growth phenotype would not be missed. 

^cNA, not applicable.