FIGURE 5.

STAT6 and YY1 bind at the IL13–1112 region in vivo. Naive peripheral blood CD4⁺ T cells from a healthy IL13–1112T heterozygote were differentiated in vitro for 2 wk under Th2 conditions. ChIP assays with an anti-STAT6, anti-YY1, or control Ab (mouse IgG1) were performed on cells (5 × 10⁷ per immunoprecipitation) cultured in the presence (+) or absence (−) of PMA and ionomycin (P/I) for 3 h. Top, Input DNA (5 or 10 ng) or immunoprecipitated DNA (one-twentieth and one-tenth of total) was used as template for PCR amplification of a 202-bp amplicon encompassing IL13-1112. Bottom, Input DNA (10 ng) or immunoprecipitated DNA (one-tenth of total) was used as template for PCR amplification of a 152-bp amplicon corresponding to CNS-1. Results are from one representative experiment of three independent immunoprecipitations.