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Representative pictures illustrate double immunostaining with Cx43 and GFAP antibodies, demonstrating the presence of gap junctions in the GLS formed by subpial astrocytes and their cytoplasmic processes. Merged pictures were created by overlaying the red, green, and blue fluorescence channels, with the blue color indicating the position of the cell nuclei. Arrowheads indicate GLS position, and P indicates pia mater. P. Scale bars = 100 μm.
