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. 2024 Oct 10;25(20):10908. doi: 10.3390/ijms252010908

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© 2024 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Southern blots to confirm the deletions of chromosomal ERG25 and 22A1 alleles. Genomic DNA samples from WT, ERG25^+/−, erg25^−/− (three clones), 22A1^+/− and 22A1^−/− +pXNG4-22A1 (two clones) parasites were digested with restriction enzymes and hybridized with radiolabeled probes for the open reading frames (top) or upstream flanking regions (bottom) of 22A1 (A) or ERG25 (B). Bands corresponding to 22A1, pXNG4-22A1, ERG25 and antibiotic-resistant genes (BSD/HYG/PAC) are indicated. Details of the Southern blots were included in Supplemental Figures S3 and S4.