Figure 4.
ERG25 is not required for proliferation or virulence in L. major. (A–C) WT, erg25−/− and erg25−/− +ERG25 promastigotes were inoculated in complete M199 media at 1.0 × 105 cells/mL and culture densities were determined daily (A). After cultures entered the stationary stage (densities > 2.0 × 107 cells/mL), percentages of round cells (B) and dead cells (C) were determined daily. (D,E) Day 3 stationary phase promastigotes were injected into the footpads of female BALB/c mice. The development of footpad lesions was monitored weekly (D) and parasite numbers in infected footpads were determined at 4 and 7 weeks (one mouse each) post-infection via limiting dilution assay (E). Error bars represent standard deviations.