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ERG25 deletion does not affect sterol composition in L. major. (A–D) Partial GC chromatograms of free sterols from WT (A), erg25−/− (B), erg25−/− +ERG25 (C) and erg25−/− +GFP-ERG25 (D) promastigotes. The peaks represented ergosterol (1), 5-dehydroepisterol (2), cholesterol (3), cholesta-5,7,24-trienol (4) and cholesta-3,5-diene standard (std). Sterol compositions and structures were indicated in (E).
