TABLE 2.
Decrease of production of RANTES, MIP-1α, MIP-1β, TNF-α, and IFN-γ and of HIV-1IIIB suppression after treatment of CTL with monensin and brefeldin Aa
| Treatment | Decrease of concn (%) of:
|
Decrease of suppression (%) of HIV-1IIIB | ||||
|---|---|---|---|---|---|---|
| RANTES | MIP-1α | MIP-1β | TNF-α | IFN-γ | ||
| Monensin | 0 | 71.6 | 70.0 | 73.1 | 99.5 | 91.5 |
| Brefeldin A | 0 | 98.0 | 98.1 | 94.5 | >99.9 | 97.5 |
a
CTL clone 15160D75 at 5 × 105 cells/ml was activated by CD3 cross-linking and treated with monensin (2 μM) or with brefeldin A (3.5 μM). Chemokine concentration or inhibition tests with supernatants were performed after 4 h of stimulation.