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ID50 and purification factors for each purification step, using 4-h supernatant of anti-CD3-stimulated bulk CD8+ cells of seropositive individuals or HIV-1-specific CTL
| Sample | ID50 (μg/ml) | Purification factor |
|---|---|---|
| Supernatant | 5,000 | 1 |
| Total heparan sulfate unbound | 770 | 7 |
| Total heparan sulfate bound | 330 | 15 |
| 350 mM heparan sulfate bound | 23.3 | 215 |
| 40-kDa heparan sulfate-bound Superdex | 5.5 | 909 |
