Fig 3. Transcriptional analysis suggests a state of chronic inflammation in R345W^+/+ RPE/choroid at 18 mo.

(A) mRNA was extracted from WT or R345W^+/+ RPE/choroid samples, reverse transcribed and probed for transcript levels of complement component 3 (C3, p ≤ 0.05), caspase 1 (Casp1), interleukin 18 (Il18), interleukin 1 beta (Il1β), interleukin 6 (Il6), nucleotide-binding oligomerization (NOD)-like receptor protein 3 (Nlrp3), complement factor H (Cfh), Efemp1 (F3), tissue inhibitor of matrix metalloproteinase 3 (Timp3) and plotted relative to β-actin (n = 5–6 mice). 2-way ANOVA. Mean ± S.D. * p < 0.05.