Figure 7. SLC35A1 and AAV capsid are colocalized with TGN46.

(A&B) HEK293 cells. SLC35A1-KO or Scramble HEK293 cells were transduced with (A) rAAV5 at MOI of 20,000 or (B) rAAV2.5T at MOI of 2,000. At 8 hpt, the cells were fixed and permeabilized, followed by immunostaining with the first antibody against indicated protein and fluorescence-conjugated secondary antibodies. (C) HAE-ALI cultures. The HAE-ALI cultures differentiated from SLC35A1-KO or Scramble CuFi-8 cells were transduced with rAAV2.5T at MOI of 20,000. At 3 dpt, the cells were fixed and permeabilized, followed by immunostaining with the first antibody against indicated protein and fluorescence-conjugated secondary antibodies. The stained cells were imaged under a confocal microscope (CSU-W1 SoRa, Nikon) at 60× with 4×SoRa magnitude (scale bar = 20 μm).