Table 4.
Secondary metabolites | Name of the test | MS | SL | MSS | SLS | EMS | ESL | HMS | HLS |
---|---|---|---|---|---|---|---|---|---|
Phenols | Neutral FeCl3 test | − | − | ++ | + | + | + | + | + |
Flavonoids | Alkaline reagent test | +++ | +++ | + | + | +++ | ++ | +++ | +++ |
Alkaloids | Mayers test | + | ++ | − | − | ++ | + | + | + |
Carbohydrates | Benedict’s test | − | − | − | − | − | ++ | − | + |
Sterols | Salkowski test | + | + | + | + | + | + | − | + |
Proteins | Biuret test | − | − | − | − | − | − | − | − |
Saponins | Foam test | +++ | +++ | − | − | − | − | + | ++ |
Terpenoids | + | + | + | + | + | + | + | + | |
Tannins | − | − | − | + | − | + | − | − | |
Fat and oils | − | − | ++ | ++ | ++ | ++ | − | − |
Note: − Negative, + Present, ++ Moderate, +++ Strongly present. MS = fresh matured stem water extract; SL = fresh stem and leaf water extract; SLS = fresh stem and leaf pet-ether extract; MSS = fresh matured stem pet-ether extract; EMS = fresh matured stem ethanol extract; ESL = fresh stem and leaf ethanol extract; HLS = fresh stem and leaf hydroalcoholic extract; HMS, fresh matured stem hydroalcoholic extract