FIG. 2.
Western blot analysis and immunoprecipitation of BTV proteins by antiserum raised against the purified VP5. (A) Western blot of purified BTV virions probed with an anti-VP5 VP5 antibody. (B) Western blot of purified BTV virion probed with anti-BTV-10 antiserum. (C) Immunoprecipitation of purified and recombinant BTV proteins by anti-VP5 (lanes 1 and 2) and anti-VP2 (lanes 3 and 4) PAbs. BHK-21 cells were infected with BTV-10 virus at an MOI of 5 and radiolabeled with [35S]methionine for 2 h at 60 h postinfection, followed by immunoprecipitation (lanes 1 and 3). Sf9 cells infected with recombinant baculoviruses expressing either VP5 (lane 2) or VP2 (lane 4) were similarly labeled and immunoprecipitated as controls. Cells were lysed with RIPA buffer, and the supernatant was immunoprecipitated with the two antibodies. Lanes 1 and 3, BTV proteins in BHK-21 cell lysates precipitated by anti-VP5 and anti-VP2 antibodies, respectively; lanes 2 and 4, recombinant VP5 and VP2 precipitated with anti-VP5 and anti-VP2 antibodies, respectively.