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. 2024 Oct 15;25(20):11094. doi: 10.3390/ijms252011094

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© 2024 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Recombinant lentiviruses pseudotyped with SARS-CoV-2 Spike delivering a Red Fluorescent Protein (Lenti-RFP-Spike). (a) Cryo-EM image of the purified Lenti-RFP-Spike imaged on Talos Arctica 200kV equipped with Gatan K2 Summit detector at a magnification of 36,000×. (b) HEK293T stably expressing human Angiotensin-Converting Enzyme 2 (HEK293T-hACE2) infected with a Lenti-RFP-Spike, fixed with 1% formaldehyde and stained with anti-spike antibodies conjugated to GFP (20× objective, zoom of 2, 400× magnification). (c) Lenti-RFP-Spike binds to the hACE2 receptor on the surface of the HEK293T-hACE2 cells to enter and express RFP in infected cells (20× objective, 40× magnification).