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. 2024 Oct 6;15(10):772. doi: 10.3390/insects15100772

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© 2024 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Genome organization and phylogenetic analysis of BiTV1. (A) The genome encodes two overlapping proteins: CP and RdRp, and the coverage of raw sequencing reads mapped to the reference genome of BiTV1. (B) Phylogenetic analysis based on the aa sequence of the full-length CP from members of different genera in the family Totiviridae, with Giardia lamblia virus in the genus Giardiavirus serving as the outgroup. The tree was generated using iqtree2 with the Q.pfam + F+R4 best-fit model with 1000 bootstraps. Bootstrap values (>60%) are shown at each node of the tree. The bar represents the genetic distance. The novel virus identified in this study is highlighted in red.