Table 1. Primer sequences and melting temperatures.
Designation | Sequence | Tm |
---|---|---|
Series A and B | ||
Limiting primers | ||
CF403 S18 | GATTATGCCTGGCACCAT | 51 |
CF402 S19 | GGATTATGCCTGGCACCAT | 54 |
CF401 S21 | CTGGATTATGCCTGGCACCAT | 57 |
CF399 S22 | CCTGGATTATGCCTGGCACCAT | 59 |
CF398 S23 | TCCTGGATTATGCCTGGCACCAT | 61 |
CF396 S25 | TTTCCTGGATTATGCCTGGCACCAT | 61 |
Excess primer | ||
CF479 A20 | TGATGACGCTTCTGTATCTA | 54 |
Series C | ||
Limiting primers | ||
CF402 S19t | TGATTATGCCTGGCACCAT | 53 |
CF401 S20 | TGGATTATGCCTGGCACCAT | 56 |
CF399 S22 | CCTGGATTATGCCTGGCACCAT | 59 |
CF392 S29 | CAGTTTTCCTGGATTATGCCTGGCACCAT | 64 |
Excess primers | ||
CF475 A16 | GACGCTTCTGTATCTA | 47 |
CF477 A18 | ATGACGCTTCTGTATCTA | 50 |
CF479 A20 | TGATGACGCTTCTGTATCTA | 54 |
CF483 A24 | GCTTTGATGACGCTTCTGTATCTA | 59 |